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- RPG gameplay in which players work their way through challenges they can level characters up with experience points.
- One featuring Bowser as a playable character, and the other revolving around the microbe-sized Mario, Luigi and Peach.
- Integrated gameplay action which the player toggles between, where Mario and Luigi must solve puzzles and challenges
- Distinctive Nintendo humor featuring the same hilarious dialogue that has been the hallmark of the Mario & Luigi series.
- Blow into the microphone or tap on the touch screen with the stylus to keep up with the action.